Microbe Stage Appendices

This page or section contains outdated information. This often happens when plans are changed after something has been written.

There used to be a more up to date google doc but it was lost. Here are the things that were saved.

Agents

Digestive Enzymes	Damages and breaks cell walls, cell capsules, membranes, and large particles (like protein, DNA, organelles, fats, dead cells)
Acidic Agent	Neutralizes alkaline environments/hazards
Alkaline Agent	Neutralizes acidic environments/hazards
Paralytic/Inhibitor	Slows down/shuts down the target organelles of the affected cell
Cyanide/Toxin	Tries to kill a cell (but not necessarily causing it to burst?)
Stimulant	Increases processing power of target organelles of the affected cell

Mutations

Mutation	Function	Source	3D Model Available	Priority	Type	Upgrades/Crossgrades	Mutation Points	ATP/s	Notes
			Y or N	1, 2, 3, or DONE. Blank means it's being discussed.
What are the different types of mutations?
Internal	A part that is placed anywhere on the cell.
External	A part that has to be placed on the edge of the cell.
Periphery	A part that that is placed as a feature of the membrane.
Sliders	Some attributes are changed via sliders. These are not placeable parts.
Sliders
Membrane Fluidity
Cell Wall	Upgrade to the membrane/cytoskeleton. Protects the cell but slows it down and makes the membrane rigid. Prevents the placement of some external organelles.	Available from start				Physical protection, agent protection, osmolysis protection, nutrient permeability
Rigid	The cell is encased by a capsule that keeps its shape.	Available from start
Semi-Fluid	The cell is able to move its membrane.	Requires Centrioles?
Fluid	The cell's membrane is very loose and flowy.	Requires Centrioles?
Osmotic Pressure
High	Adapted for deep ocean.
Low	Adapted for shallow ocean
DNA
Nucleoid	The cluster of DNA in prokaryotic cells. The starting organelle. Can only have one, cannot remove.	Starts in cell					n/a	0
Plasmids	Allow horizontal gene transfer with cells that you ingest. Upgrading it allows you to perform Horizontal Gene Transfer with other cells of your species.	Available from start
Nucleic Core	Includes the nucleus, endoplasmic reticulum, and golgi apparatus. Enables new types of agents and organelles. Changes the type of the cell from a prokaryote into a eukaryote. Can only have one, cannot remove.	Requires Vacuole	Y	Done			100	-15	Could trigger a cinematic in which we see the cell performing endocytosis or pinocytosis, and thus, forming the said organelles.
Conjugal Nuclei	Enables sexual reproduction.	Requires Nucleic Core					75	-10
Macronucleus	Upgrade to a nucleus? Gives an MP bonus but is a hassle to drag around?
Cytoskeleton
Motility Cilia	Small movement with good manueverability using small amounts of ATP.	Available from start				Sensory Cilia, Directed Cilia, increased movement speed	33	-2 when active
Sensory Cilia	If linked to a receptor protein, increases its efficacy.	Available from start				Motility Cilia, Directed Cilia, increased boost	33	-5 when active
Directed Cilia	Extends the reach of your cell when swimming through compound / agent clouds so you gather both from a larger area.	Available from start				Motility Cilia, Sensory Cilia, increased boost	33	-2 when active
Flagellum	Large unidirectional movement with low manueverability using large amounts of ATP.	Available from start	Y	Done		Pilus, Injector Pilus, increased movement speed	50	-5 when active
Pilus (1)	A large and stiff physical protrusion that can be used to ram and puncture cells. Does mellee damage.	Available from start	Y	Done		Flagellum, Injector Pilus, increased movement speed	66		I marked these a 1,2,3,4 to specify how they might be one multifunctional thing or a pilus might be specialised by adding an agent gland or a vacuole or contracile fibers.
Injector Pilus (2)	Can inject agents into other cells.	Available from start				Flagellum, Pilus, increased movement speed	66	-5 when active
Straw Pilus (3)	Can suck compounds out of other cells. Can suck DNA (+ mutation points) from your own species.							-5 when active
Spear Pilus (4)	A pilus which can shoot out violently to do extra damage.							-15 when active
Grappling Hook
Lamellipodia	Allow crawling and attachment to surfaces.	Requires semi-fluid or fluid membrane					50
Contractile Fibers	Allows the cell to extend larger pseudopods (boosts ameboid movement). Later increases efficacy as a muscle cell in Multicellular.	Requires semi-fluid or fluid membrane					50	-10
Membrane
Cytoplasm	Provides cell structure, provides a bit of storage, and allows simple process of fermentation. Glucose => 8 ATP (Oxygen)	Available from start		Done			25	2
Cell Wall	Upgrade to the membrane/cytoskeleton. Protects the cell but slows it down and makes the membrane rigid. Prevents the placement of some external organelles.	Available from start		Done		Physical protection, agent protection, osmolysis protection, nutrient permeability
Microvilli/Folded Membrane/Membrane Invaginations	Increases the surface area of the cell. 10% bonus to compounds gathered from clouds, 10% increase in agents gathered.	Requires a semi-fluid or fluid membrane
Vacuole	Stores compounds (or agents using some ATP. Can choose what to store. Maximum storage capacity based on number of hexes. Can increase cell rigidity by maintaining turgor pressure.)	Available from start	Y	Done		Increased storage space	33	-2
Contractile Vacuole	Allows a cell to change its pressure tolerance by filling or emptying its contractile vacuole.	Requires Vacuole				Increased pressure range		-5
Agentosome/Agent Secretor	Secretes agents into the environment using no ATP. Must be assigned a hotkey and a single type of agent. Can be placed over cell wall. Only organelle to be able to do that?	Available from start				Predatory pilus		-10 when active
Bioluminescent Dot/Protein	Allows a cell to emit bioluminescence. (Alternative to vacuoles containing bioluminescent agents.) Lights up with constant ATP draining. (Provides “sunlight” for photosynthesizers.)	Requires Vacuole or Assimilation of bioluminescent cell		1				-10 when active
Microbial Jet									https://www.newscientist.com/article/dn8933-bacteria-use-slime-jets-to-get-around/
Proteins
Antifreeze Protein	50% leff damage from ice.							-5
Heat Resistant Protein	50% less damage from heat spots.							-5
Lysosomes	Allows ingested cells and macromolecules/large particles to be digested much more efficiently. 5% reduction in compounds required for reproduction.	Requires a Nucleic Core						-5
Peroxisomes	10% agent resistance	Requires a Nucleic Core						-5
Photoreceptor Protein	Allows the cell to detect light
Eyespot	Provides a vision cone and if you scan that cone over the edge of the screen it will highlight lightspots which are close by but offscreen.
Chemoreceptor Protein	Allows the cell to detect chemicals		Y
Thermoreceptor Protein
Electroreceptor? Mechanoreceptor?
Adhesion Proteins	Allows the cell to have its daughter cells stay attached when produced and form a multicellular colony.	Requires a Nucleic Core	Y
Pigment Protein	Protects the cell from solar radiation and provides a colour. 50% reduction in light spot damage.
Signal Protein	Summons members of target species.	Requires a Nucleic Core
Immune Protein	? Digests harmful ingested agents? Blocks viruses?
Nitrogenase (Nitrogen Fixing Protein)	Performs Nitrogen Fixation: => Ammonia Upgrades to nitrogen fixing plastid.		Y	Done
Aerobic Respiration Protein	Performs aerobic respiration to produce ATP: Glucose => 18 ATP (Oxygen) Upgrades to mitochondrion if upgraded to Eukaryote.	Requires to be a Prokaryote	Y	Done				36
Thylakoid/Photosynthetic Protein?	Performs photosynthesis: => Glucose (CO2, Sunlight). Upgrades to chloroplast.		Y	Done
Melanin	Performs radiosynthesis
Endosymbionts/Plastids
Mitochondrion	Performs aerobic respiration to produce ATP: Glucose => 36 ATP (Oxygen)	Requires a Nucleic Core	Y	Done		Increase efficiency (ATP output), increase reaction speed		36
Chloroplast	Performs photosynthesis: => Glucose (CO2, Sunlight)	Requires a Nucleic Core	Y	Done		Increase efficiency (glucose output), increase reaction speed		-25	While C4 (increased chloroplast efficiency) enhances the efficiency of RuBisCO, the concentration of carbon is highly energy intensive. This means that C4 plants only have an advantage over C3 organisms in certain conditions: namely, high temperatures and low rainfall. C4 plants also need high levels of sunlight to thrive.[29] Models suggest that, without wildfires removing shade-casting trees and shrubs, there would be no space for C4 plants.
Iron Respiring Protein			Y	Done
Chemoplast / Hydrogen Sulfide Oxidizing Plastid	Performs Hydrogen Sulfide Oxidization: Hydrogen Sulfide => Glucose		Y	Done
Nitrogen Fixing Plastid	Performs Nitrogen Fixation: => Ammonia		Y	Done
Tannosome	Any cell using a straw pilus on this cell takes damage.
Thermoplast	???		Y

Biomes

Biome	Sunlight (% Intensity)	Temperature (°C)	Energy Source	Currents	Salinity (%)	Pressure (atm)	pH	Compounds (Clouds)	Compounds (Gasses) (%)	Visuals	Notes
Hydrothermal Vent, Depth [3, 5]km	0	400 near the vents, 2-4 a few meters away	Hydrogen Sulfide, falling detritus	Medium	None	High	Neutral	Hydrogen Sulfide (High), Iron (High), Manganese (High), Methane (High), Ammonia (High)	Oxygen (High)	Dark blues and blacks mixed with streaks of grey and white and red/orange/brown.	The hydrothermal vent is assumed to be in the deep ocean, which affects many of its variables. Rocky surfaces. Organic detritus falling from above?
Ocean floor / Abyssopelagic, Depth [4, 6]km	0, totally dark, bioluminscence	[2, 3]	Falling detritus		None to Low	Very high				Same as Deep ocean
Deep Ocean / Bathypelagic, Depth [1, 4[km	0 and it's totally dark, bioluminscence	[0, 10[	Limited Falling detritus	Weak to none	Low	High	Neutral		Oxygen (High)	Dark blues and blacks, very dark without bioluminesnce	Also known as Abyss or Benthic Ocean. Oxygen levels are high because of cold oxygenated water flowing in from polar melting. Salinity is lower and currents are weaker in the deep ocean (confirm this). At these depths water pressure is very high. Organic detritus falling from above?
Mid Ocean / Mesopelagic, Depth [200, 1000[m	0 but you can see	[10, +[	Limited falling detritus		Low to Medium	Medium				Medium blue
Ocean Surface / Epipelagic, Depth [0, 200[m	50-80	]-, +[	Sunlight		High	Low				light blue, quite bright, easy to see
Coastal Shelf / Shallow Ocean Depth [0, 140[m	50-80	10?	Sunlight	Medium	High	Low	Neutral	Iron (High), Manganese (High)	Oxygen (Medium)	Lighter blues and greens and whites.	Also known as Coastal Ocean. Near the surface of the ocean the sunlight penetrates enough to provide warmth and light, but also deadly UV radiation. Oxygen here is close enough to the atmosphere that it’s level is more in equilibrium with the atmospheric level. Salinity is high near the ocean surface because of a high amount of evaporation, and pressure is low. Dissolved minerals are common along the coast. Occasional rocky surfaces
Tidepool	75-100	14?	Overly intense sunlight	Strong	High	Low	Neutral	Iron (High), Manganese (High)	Oxygen (Medium)	Lighter blues and greens/greys/yellows/browns.	The tidepool is completely exposed to the warmth and light, but also deadly UV radiation, of the sun (which could potentially lead to extremely fast mutations). Oxygen here is close enough to the atmosphere that it’s level is more in equilibrium with the atmospheric level. Salinity is high near the ocean surface because of a high amount of evaporation, and pressure is low. Dissolved minerals are common along the coast. What makes the tidepool distinct from the Coastal Shelf biome is that it is filled with strong currents from the waves, and a high amount of nutrients and minerals carried by the currents to the shoreline. Many small rocks.
Underwater Cave	0, totally dark, bioluminescence		Hydrogen Sulfide							Dark grey?
Polar Sea	30%, enough for slow photosynthesis		Sunlight							Ice white and blue
Estuary, Depth [0, 300]m	50-80%		Sunlight + maybe minerals washing down the river							Could be brown for muddy river bottom?
To be determined:
Microbial Mat/Stromalotite?
Hadopelagic zone

Compounds

Compound	Icon	Colour	Weight (g/mol)	Description	Involved in
Clouds	Icon
Ammonia	Three atoms connected to a central atom	Orange-ish yellow	17
Glucose	Sugar cube	White	180
Phosphate	TBD	Pale Purple	95
Iron	TBD	Orange	56
Hydrogen Sulfide	TBD	Pale greenish yellow	34
Calcium	Bone	Pale cream	40	Rock surfaces are gradually worn away to produce calcium?
Calcium Carbonate	Bricks	Cream	100	Solidifies on release into the environment, bonding with nearby carbonate particles to create solid structures.
Gasses
Carbon Dioxide	Three connected atoms		44
Oxygen	Bubbles		32
Nitrogen	TBD		28
Methane	Gas		16
Carbon Monoxide	TBD		28
Hydrogen	Flame		2
Unique
ATP	Lightning bolt	Green, lol.	507	ATP cannot be found in the environment and has no weight. It is simply produced and used up whenever energy is transferred within a cell.
Water	Water droplet		18	Water is found throughout the environment at all times, both inside and outside cells. It provides a constant influx into the compounds system.
Yet to be discussed:
Hydrogen Cyanide	Two medical pills and a death skull, side by side	Brown	27
Lactic Acid? Ethanol?	Muscles? Alcohol?
Sulfur	Cristals/Powder of sulfur or S2	Yellow	64
Sulfuric Acid		Yellow?	98
Organic Acid	Bottle of vinegar	Pale green	60	Based on acetic acid.
Nitrate?	N2 bottle		62
Protein	Meat	Bloody red	6500	All proteins are covered by a single compound for the sake of simplicity, made by the same process using the same reactants.
Lipids	Fatty and oily school cookies, lol. So fatty and oily that you must eat them with a spoon.	Pale orange	860
DNA
Starch/Polysaccharide
Fatty Acids		Pale yellow	256	Used palmitic acid as a model.
Amino acids	Number 20	Pale pink	130	Based on (MaxMolarWeight - MinMolarWeight)/2 and some rounding for making the numbers more beautiful
*Enzymes (and coenzymes)				Probably replaced by proteins
Uranium	Radioactive warning icon	Lime green	238	I have seen in a documentary that some bacteria use uranium as a shield against radiations.
Glycerol			92

Music

Track	Artist	Scene	Link
Vigeo (Main Theme)		Title Screen	https://revolutionarygames.bandcamp.com/track/vigeo-main-theme
Vireo (Extended Main Theme)		Promotional Material; Credits	https://revolutionarygames.bandcamp.com/track/vireo-extended-main-theme
Vigent (Main Theme Suite)		Extras
Genesis		Opening Cutscene	https://revolutionarygames.bandcamp.com/track/genesis
Into the Tidepool		Loading Screen	https://revolutionarygames.bandcamp.com/track/into-the-tidepool
Cellular Exploration		Loading Screen	https://revolutionarygames.bandcamp.com/track/cellular-exploration
Hydrothermal		Loading Screen	https://revolutionarygames.bandcamp.com/track/hydrothermal
Silence Underwater		Environment	https://revolutionarygames.bandcamp.com/track/silence-underwater
Unicellular Organisms		Environment	https://revolutionarygames.bandcamp.com/track/unicellular-organisms
Microscopic		Environment	https://revolutionarygames.bandcamp.com/track/microscopic
Tidepool Landscapes		Environment	https://revolutionarygames.bandcamp.com/track/tidepool-landscapes
Angels in a Bubble		Environment	https://revolutionarygames.bandcamp.com/track/angels-in-a-bubble
The Saga of the Puddle		Environment	https://revolutionarygames.bandcamp.com/track/the-saga-of-the-puddle
Flagellum		Editor	https://revolutionarygames.bandcamp.com/track/flagellum
Mitosis in Progress		Editor	https://revolutionarygames.bandcamp.com/track/mitosis-in-progress
The Cradle of Life		Editor	https://revolutionarygames.bandcamp.com/track/the-cradle-of-life
Small Beginnings		Editor	https://revolutionarygames.bandcamp.com/track/small-beginnings
A Microbe’s Lullaby		Editor	https://revolutionarygames.bandcamp.com/track/a-microbes-lullaby

Processes

Name	Requires	Produces	Catalysts	Location	Details	References
Respiration
Fermentation	1 Glucose	2 Lactic Acid + 2 Water + 2 ATP/ 2 Ethanol + 2 Carbon Dioxide + 2 ATP		Cytoplasm		Wikipedia
Aerobic Respiration	1 Glucose + 6 Oxygen + 2 ATP	6 Carbon Dioxide + 6 Water + 38 ATP		Mitochondrion or Aerobic Respiration Protein
Iron Respiration	2 Fe(III) + 2 I(-)	2 Fe(II) + I2		Cytoplasm	2 I(-) can be replaced by Sn or Zn/I2 can be replaced by Sn(2+) or Zn(2+)	http://chemequations.com and wikipedia
Manganese Respiration	Mn(IV) + 2 I(-)	Mn(II) + I2		Cytoplasm	problem : where is ATP	http://chemequations.com, wikipedia and a kind of report
Methanogenesis	1 Carbon Dioxide + 4 Hydrogen	1 Methane + 2 Water	Proteins (enzymes)	Methanoplast or Methanogenesis Protein?	where is atp	Wikipedia
Methane respiration/oxydation?	6 Methane + 6 Water + 6 Oxygen (ultimate satanism!)	1 Glucose		Methanoplast or Methanogenesis Protein?	where is atp + could be combined with methanogenesis (maybe in the same organelle to make it useful)	chemequations.com
Biosynthesis
Anoxygenic Photosynthesis	6 Carbon Dioxide + 12 Hydrogen Sulfide + Sunlight	1 Glucose + 6 Sulfur (S2) + 6 Water		Anoxygenic chloroplast		http://chemequations.com and wikipedia
Oxygenic Photosynthesis	6 Carbon Dioxide + 6 Water + Sunlight	1 Glucose + 6 Oxygen		Chloroplast or Photosynthetic Protein
Growth
Amino Acid Synthesis	2 Glucose + 2 Ammonia	1 Amino Acid		Ribosomes?	Keep it simple or the player will kill you.
Fatty Acid Synthesis	2 Glucose	1 Fatty Acid		Cytoplasm	Keep it simple or the player will kill you.
Nucleic Acid Synthesis	1 Glucose + 1 Ammonia + 1 Phosphate	1 Nucleic acid or nucleotide		Nucleoid/Nucleus	Keep it simple or the player will kill you.
Membrane repair (health regen)	Fatty Acid + Phosphate maybe?	1 or 2 HP
Agent Synthesis		ER
Carbonate Synthesis	2Ca + 3(CO2) – > 2(CaCO3)
Locked-Up Conversion	Any compound -> Locked-up version	Cytoplasm

Natural Events

Event	Triggers	Effects	Scale
Oxygenation Event	Once a species has evolved oxygenic photosynthesis, an Oxygenation Event will take place between 5-8 generations. More species evolving oxygenic photosynthesis shortens this time.	Makes oxygen a High abundance compound in Deep Ocean, Hydrothermal Vent,	Global
Ocean Acidification			Global
Snowball Earth/Ice Age			Global

SFX

Sound	Scene	Type
Agent Secretion	Environment	Environmental; one shot
Agent Damage	Environment	Interface; repeat (plays once every set period for the duration a microbe takes damage)
Agent Inhibition	Environment	Interface; repeat (plays once every set period for the duration a microbe is inhibited)
Ambience	Environment/Editor	Interface; continuous
Button Click Unavailable	All	Interface; one shot
Button Hover/Click	All	Interface; one shot
Disaster	Environment	Interface; one shot; various types for different disasters, some last longer
Endocytosis Success	Environment	Interface; one shot
Engulfment	Environment	Environmental; one shot
Extinction (Game Over)	Environment	Interface; one shot; likely to be somewhat musical in nature
Insufficient Mutation Points	Editor	Interface; one shot
Microbe Bonding	Environment	Environmental; one shot
Microbe Death	Environment	Environmental; one shot
Microbe Division	Environment	Environmental; one shot
Microbe-Microbe Collision	Environment	Environmental; one shot; volume scales with impact speed
Microbe-Surface Collision	Environment	Environmental; one shot; volume scales with impact speed
Movement 1	Environment	Environmental; one shot; plays with substantial change in linear or angular momentum
Movement 2	Environment	Environmental; one shot; plays with substantial change in linear or angular momentum
Movement 3	Environment	Environmental; one shot; plays with substantial change in linear or angular momentum
Multicellular (Victory)	Environment	Interface; one shot; likely to be somewhat musical in nature
Organelle Collection	Environment	Interface; one shot; overridden by endocytosis successful
Pilus Stab	Environment	Environmental; one shot
Selection/Placement	Editor	Interface; one shot
Selection/Placement Invalid	Editor	Interface; one shot; overridden by insufficient Mutation Points sound
Surface-Surface Collision	Environment	Environmental; one shot; Environmental; one shot; volume scales with impact speed