Microbe Stage Appendices

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This page or section contains outdated information. This often happens when plans are changed after something has been written.

There used to be a more up to date google doc but it was lost. Here are the things that were saved.

Agents

Digestive Enzymes Damages and breaks cell walls, cell capsules, membranes, and large particles (like protein, DNA, organelles, fats, dead cells)
Acidic Agent Neutralizes alkaline environments/hazards
Alkaline Agent Neutralizes acidic environments/hazards
Paralytic/Inhibitor Slows down/shuts down the target organelles of the affected cell
Cyanide/Toxin Tries to kill a cell (but not necessarily causing it to burst?)
Stimulant Increases processing power of target organelles of the affected cell

Mutations

Mutation Function Source 3D Model Available Priority Type Upgrades/Crossgrades Mutation Points ATP/s Notes
Y or N 1, 2, 3, or DONE. Blank means it's being discussed.
What are the different types of mutations?
Internal A part that is placed anywhere on the cell.
External A part that has to be placed on the edge of the cell.
Periphery A part that that is placed as a feature of the membrane.
Sliders Some attributes are changed via sliders. These are not placeable parts.
Sliders
Membrane Fluidity
Cell Wall Upgrade to the membrane/cytoskeleton. Protects the cell but slows it down and makes the membrane rigid. Prevents the placement of some external organelles. Available from start Physical protection, agent protection, osmolysis protection, nutrient permeability
Rigid The cell is encased by a capsule that keeps its shape. Available from start
Semi-Fluid The cell is able to move its membrane. Requires Centrioles?
Fluid The cell's membrane is very loose and flowy. Requires Centrioles?
Osmotic Pressure
High Adapted for deep ocean.
Low Adapted for shallow ocean
DNA
Nucleoid The cluster of DNA in prokaryotic cells. The starting organelle. Can only have one, cannot remove. Starts in cell n/a 0
Plasmids Allow horizontal gene transfer with cells that you ingest. Upgrading it allows you to perform Horizontal Gene Transfer with other cells of your species. Available from start
Nucleic Core Includes the nucleus, endoplasmic reticulum, and golgi apparatus. Enables new types of agents and organelles. Changes the type of the cell from a prokaryote into a eukaryote. Can only have one, cannot remove. Requires Vacuole Y Done 100 -15 Could trigger a cinematic in which we see the cell performing endocytosis or pinocytosis, and thus, forming the said organelles.
Conjugal Nuclei Enables sexual reproduction. Requires Nucleic Core 75 -10
Macronucleus Upgrade to a nucleus? Gives an MP bonus but is a hassle to drag around?
Cytoskeleton
Motility Cilia Small movement with good manueverability using small amounts of ATP. Available from start Sensory Cilia, Directed Cilia, increased movement speed 33 -2 when active
Sensory Cilia If linked to a receptor protein, increases its efficacy. Available from start Motility Cilia, Directed Cilia, increased boost 33 -5 when active
Directed Cilia Extends the reach of your cell when swimming through compound / agent clouds so you gather both from a larger area. Available from start Motility Cilia, Sensory Cilia, increased boost 33 -2 when active
Flagellum Large unidirectional movement with low manueverability using large amounts of ATP. Available from start Y Done Pilus, Injector Pilus, increased movement speed 50 -5 when active
Pilus (1) A large and stiff physical protrusion that can be used to ram and puncture cells. Does mellee damage. Available from start Y Done Flagellum, Injector Pilus, increased movement speed 66 I marked these a 1,2,3,4 to specify how they might be one multifunctional thing or a pilus might be specialised by adding an agent gland or a vacuole or contracile fibers.
Injector Pilus (2) Can inject agents into other cells. Available from start Flagellum, Pilus, increased movement speed 66 -5 when active
Straw Pilus (3) Can suck compounds out of other cells. Can suck DNA (+ mutation points) from your own species. -5 when active
Spear Pilus (4) A pilus which can shoot out violently to do extra damage. -15 when active
Grappling Hook
Lamellipodia Allow crawling and attachment to surfaces. Requires semi-fluid or fluid membrane 50
Contractile Fibers Allows the cell to extend larger pseudopods (boosts ameboid movement). Later increases efficacy as a muscle cell in Multicellular. Requires semi-fluid or fluid membrane 50 -10
Membrane
Cytoplasm Provides cell structure, provides a bit of storage, and allows simple process of fermentation. Glucose => 8 ATP (Oxygen) Available from start Done 25 2
Cell Wall Upgrade to the membrane/cytoskeleton. Protects the cell but slows it down and makes the membrane rigid. Prevents the placement of some external organelles. Available from start Done Physical protection, agent protection, osmolysis protection, nutrient permeability
Microvilli/Folded Membrane/Membrane Invaginations Increases the surface area of the cell. 10% bonus to compounds gathered from clouds, 10% increase in agents gathered. Requires a semi-fluid or fluid membrane
Vacuole Stores compounds (or agents using some ATP. Can choose what to store. Maximum storage capacity based on number of hexes. Can increase cell rigidity by maintaining turgor pressure.) Available from start Y Done Increased storage space 33 -2
Contractile Vacuole Allows a cell to change its pressure tolerance by filling or emptying its contractile vacuole. Requires Vacuole Increased pressure range -5
Agentosome/Agent Secretor Secretes agents into the environment using no ATP. Must be assigned a hotkey and a single type of agent. Can be placed over cell wall. Only organelle to be able to do that? Available from start Predatory pilus -10 when active
Bioluminescent Dot/Protein Allows a cell to emit bioluminescence. (Alternative to vacuoles containing bioluminescent agents.) Lights up with constant ATP draining. (Provides “sunlight” for photosynthesizers.) Requires Vacuole or Assimilation of bioluminescent cell 1 -10 when active
Microbial Jet https://www.newscientist.com/article/dn8933-bacteria-use-slime-jets-to-get-around/
Proteins
Antifreeze Protein 50% leff damage from ice. -5
Heat Resistant Protein 50% less damage from heat spots. -5
Lysosomes Allows ingested cells and macromolecules/large particles to be digested much more efficiently. 5% reduction in compounds required for reproduction. Requires a Nucleic Core -5
Peroxisomes 10% agent resistance Requires a Nucleic Core -5
Photoreceptor Protein Allows the cell to detect light
Eyespot Provides a vision cone and if you scan that cone over the edge of the screen it will highlight lightspots which are close by but offscreen.
Chemoreceptor Protein Allows the cell to detect chemicals Y
Thermoreceptor Protein
Electroreceptor? Mechanoreceptor?
Adhesion Proteins Allows the cell to have its daughter cells stay attached when produced and form a multicellular colony. Requires a Nucleic Core Y
Pigment Protein Protects the cell from solar radiation and provides a colour. 50% reduction in light spot damage.
Signal Protein Summons members of target species. Requires a Nucleic Core
Immune Protein ? Digests harmful ingested agents? Blocks viruses?
Nitrogenase (Nitrogen Fixing Protein) Performs Nitrogen Fixation: => Ammonia Upgrades to nitrogen fixing plastid. Y Done
Aerobic Respiration Protein Performs aerobic respiration to produce ATP: Glucose => 18 ATP (Oxygen) Upgrades to mitochondrion if upgraded to Eukaryote. Requires to be a Prokaryote Y Done 36
Thylakoid/Photosynthetic Protein? Performs photosynthesis: => Glucose (CO2, Sunlight). Upgrades to chloroplast. Y Done
Melanin Performs radiosynthesis
Endosymbionts/Plastids
Mitochondrion Performs aerobic respiration to produce ATP: Glucose => 36 ATP (Oxygen) Requires a Nucleic Core Y Done Increase efficiency (ATP output), increase reaction speed 36
Chloroplast Performs photosynthesis: => Glucose (CO2, Sunlight) Requires a Nucleic Core Y Done Increase efficiency (glucose output), increase reaction speed -25 While C4 (increased chloroplast efficiency) enhances the efficiency of RuBisCO, the concentration of carbon is highly energy intensive. This means that C4 plants only have an advantage over C3 organisms in certain conditions: namely, high temperatures and low rainfall. C4 plants also need high levels of sunlight to thrive.[29] Models suggest that, without wildfires removing shade-casting trees and shrubs, there would be no space for C4 plants.
Iron Respiring Protein Y Done
Chemoplast / Hydrogen Sulfide Oxidizing Plastid Performs Hydrogen Sulfide Oxidization: Hydrogen Sulfide => Glucose Y Done
Nitrogen Fixing Plastid Performs Nitrogen Fixation: => Ammonia Y Done
Tannosome Any cell using a straw pilus on this cell takes damage.
Thermoplast ??? Y

Biomes

Biome Sunlight (% Intensity) Temperature (°C) Energy Source Currents Salinity (%) Pressure (atm) pH Compounds (Clouds) Compounds (Gasses) (%) Visuals Notes
Hydrothermal Vent, Depth [3, 5]km 0 400 near the vents, 2-4 a few meters away Hydrogen Sulfide, falling detritus Medium None High Neutral Hydrogen Sulfide (High), Iron (High), Manganese (High), Methane (High), Ammonia (High) Oxygen (High) Dark blues and blacks mixed with streaks of grey and white and red/orange/brown. The hydrothermal vent is assumed to be in the deep ocean, which affects many of its variables. Rocky surfaces. Organic detritus falling from above?
Ocean floor / Abyssopelagic, Depth [4, 6]km 0, totally dark, bioluminscence [2, 3] Falling detritus None to Low Very high Same as Deep ocean
Deep Ocean / Bathypelagic, Depth [1, 4[km 0 and it's totally dark, bioluminscence [0, 10[ Limited Falling detritus Weak to none Low High Neutral Oxygen (High) Dark blues and blacks, very dark without bioluminesnce Also known as Abyss or Benthic Ocean. Oxygen levels are high because of cold oxygenated water flowing in from polar melting. Salinity is lower and currents are weaker in the deep ocean (confirm this). At these depths water pressure is very high. Organic detritus falling from above?
Mid Ocean / Mesopelagic, Depth [200, 1000[m 0 but you can see [10, +[ Limited falling detritus Low to Medium Medium Medium blue
Ocean Surface / Epipelagic, Depth [0, 200[m 50-80 ]-, +[ Sunlight High Low light blue, quite bright, easy to see
Coastal Shelf / Shallow Ocean Depth [0, 140[m 50-80 10? Sunlight Medium High Low Neutral Iron (High), Manganese (High) Oxygen (Medium) Lighter blues and greens and whites. Also known as Coastal Ocean. Near the surface of the ocean the sunlight penetrates enough to provide warmth and light, but also deadly UV radiation. Oxygen here is close enough to the atmosphere that it’s level is more in equilibrium with the atmospheric level. Salinity is high near the ocean surface because of a high amount of evaporation, and pressure is low. Dissolved minerals are common along the coast. Occasional rocky surfaces
Tidepool 75-100 14? Overly intense sunlight Strong High Low Neutral Iron (High), Manganese (High) Oxygen (Medium) Lighter blues and greens/greys/yellows/browns. The tidepool is completely exposed to the warmth and light, but also deadly UV radiation, of the sun (which could potentially lead to extremely fast mutations). Oxygen here is close enough to the atmosphere that it’s level is more in equilibrium with the atmospheric level. Salinity is high near the ocean surface because of a high amount of evaporation, and pressure is low. Dissolved minerals are common along the coast. What makes the tidepool distinct from the Coastal Shelf biome is that it is filled with strong currents from the waves, and a high amount of nutrients and minerals carried by the currents to the shoreline. Many small rocks.
Underwater Cave 0, totally dark, bioluminescence Hydrogen Sulfide Dark grey?
Polar Sea 30%, enough for slow photosynthesis Sunlight Ice white and blue
Estuary, Depth [0, 300]m 50-80% Sunlight + maybe minerals washing down the river Could be brown for muddy river bottom?
To be determined:
Microbial Mat/Stromalotite?
Hadopelagic zone

Compounds

Compound Icon Colour Weight (g/mol) Description Involved in
Clouds Icon
Ammonia Three atoms connected to a central atom Orange-ish yellow 17
Glucose Sugar cube White 180
Phosphate TBD Pale Purple 95
Iron TBD Orange 56
Hydrogen Sulfide TBD Pale greenish yellow 34
Calcium Bone Pale cream 40 Rock surfaces are gradually worn away to produce calcium?
Calcium Carbonate Bricks Cream 100 Solidifies on release into the environment, bonding with nearby carbonate particles to create solid structures.
Gasses
Carbon Dioxide Three connected atoms 44
Oxygen Bubbles 32
Nitrogen TBD 28
Methane Gas 16
Carbon Monoxide TBD 28
Hydrogen Flame 2
Unique
ATP Lightning bolt Green, lol. 507 ATP cannot be found in the environment and has no weight. It is simply produced and used up whenever energy is transferred within a cell.
Water Water droplet 18 Water is found throughout the environment at all times, both inside and outside cells. It provides a constant influx into the compounds system.
Yet to be discussed:
Hydrogen Cyanide Two medical pills and a death skull, side by side Brown 27
Lactic Acid? Ethanol? Muscles? Alcohol?
Sulfur Cristals/Powder of sulfur or S2 Yellow 64
Sulfuric Acid Yellow? 98
Organic Acid Bottle of vinegar Pale green 60 Based on acetic acid.
Nitrate? N2 bottle 62
Protein Meat Bloody red 6500 All proteins are covered by a single compound for the sake of simplicity, made by the same process using the same reactants.
Lipids Fatty and oily school cookies, lol. So fatty and oily that you must eat them with a spoon. Pale orange 860
DNA
Starch/Polysaccharide
Fatty Acids Pale yellow 256 Used palmitic acid as a model.
Amino acids Number 20 Pale pink 130 Based on (MaxMolarWeight - MinMolarWeight)/2 and some rounding for making the numbers more beautiful
*Enzymes (and coenzymes) Probably replaced by proteins
Uranium Radioactive warning icon Lime green 238 I have seen in a documentary that some bacteria use uranium as a shield against radiations.
Glycerol 92

Music

Track Artist Scene Link
Vigeo (Main Theme) Title Screen https://revolutionarygames.bandcamp.com/track/vigeo-main-theme
Vireo (Extended Main Theme) Promotional Material; Credits https://revolutionarygames.bandcamp.com/track/vireo-extended-main-theme
Vigent (Main Theme Suite) Extras
Genesis Opening Cutscene https://revolutionarygames.bandcamp.com/track/genesis
Into the Tidepool Loading Screen https://revolutionarygames.bandcamp.com/track/into-the-tidepool
Cellular Exploration Loading Screen https://revolutionarygames.bandcamp.com/track/cellular-exploration
Hydrothermal Loading Screen https://revolutionarygames.bandcamp.com/track/hydrothermal
Silence Underwater Environment https://revolutionarygames.bandcamp.com/track/silence-underwater
Unicellular Organisms Environment https://revolutionarygames.bandcamp.com/track/unicellular-organisms
Microscopic Environment https://revolutionarygames.bandcamp.com/track/microscopic
Tidepool Landscapes Environment https://revolutionarygames.bandcamp.com/track/tidepool-landscapes
Angels in a Bubble Environment https://revolutionarygames.bandcamp.com/track/angels-in-a-bubble
The Saga of the Puddle Environment https://revolutionarygames.bandcamp.com/track/the-saga-of-the-puddle
Flagellum Editor https://revolutionarygames.bandcamp.com/track/flagellum
Mitosis in Progress Editor https://revolutionarygames.bandcamp.com/track/mitosis-in-progress
The Cradle of Life Editor https://revolutionarygames.bandcamp.com/track/the-cradle-of-life
Small Beginnings Editor https://revolutionarygames.bandcamp.com/track/small-beginnings
A Microbe’s Lullaby Editor https://revolutionarygames.bandcamp.com/track/a-microbes-lullaby

Processes

Name Requires Produces Catalysts Location Details References
Respiration
Fermentation 1 Glucose 2 Lactic Acid + 2 Water + 2 ATP/ 2 Ethanol + 2 Carbon Dioxide + 2 ATP Cytoplasm Wikipedia
Aerobic Respiration 1 Glucose + 6 Oxygen + 2 ATP 6 Carbon Dioxide + 6 Water + 38 ATP Mitochondrion or Aerobic Respiration Protein
Iron Respiration 2 Fe(III) + 2 I(-) 2 Fe(II) + I2 Cytoplasm 2 I(-) can be replaced by Sn or Zn/I2 can be replaced by Sn(2+) or Zn(2+) http://chemequations.com and wikipedia
Manganese Respiration Mn(IV) + 2 I(-) Mn(II) + I2 Cytoplasm problem : where is ATP http://chemequations.com, wikipedia and a kind of report
Methanogenesis 1 Carbon Dioxide + 4 Hydrogen 1 Methane + 2 Water Proteins (enzymes) Methanoplast or Methanogenesis Protein? where is atp Wikipedia
Methane respiration/oxydation? 6 Methane + 6 Water + 6 Oxygen (ultimate satanism!) 1 Glucose Methanoplast or Methanogenesis Protein? where is atp + could be combined with methanogenesis (maybe in the same organelle to make it useful) chemequations.com
Biosynthesis
Anoxygenic Photosynthesis 6 Carbon Dioxide + 12 Hydrogen Sulfide + Sunlight 1 Glucose + 6 Sulfur (S2) + 6 Water Anoxygenic chloroplast http://chemequations.com and wikipedia
Oxygenic Photosynthesis 6 Carbon Dioxide + 6 Water + Sunlight 1 Glucose + 6 Oxygen Chloroplast or Photosynthetic Protein
Growth
Amino Acid Synthesis 2 Glucose + 2 Ammonia 1 Amino Acid Ribosomes? Keep it simple or the player will kill you.
Fatty Acid Synthesis 2 Glucose 1 Fatty Acid Cytoplasm Keep it simple or the player will kill you.
Nucleic Acid Synthesis 1 Glucose + 1 Ammonia + 1 Phosphate 1 Nucleic acid or nucleotide Nucleoid/Nucleus Keep it simple or the player will kill you.
Membrane repair (health regen) Fatty Acid + Phosphate maybe? 1 or 2 HP
Agent Synthesis ER
Carbonate Synthesis 2Ca + 3(CO2) – > 2(CaCO3)
Locked-Up Conversion Any compound -> Locked-up version Cytoplasm

Natural Events

Event Triggers Effects Scale
Oxygenation Event Once a species has evolved oxygenic photosynthesis, an Oxygenation Event will take place between 5-8 generations. More species evolving oxygenic photosynthesis shortens this time. Makes oxygen a High abundance compound in Deep Ocean, Hydrothermal Vent, Global
Ocean Acidification Global
Snowball Earth/Ice Age Global

SFX

(✓) - Done/implemented.

(+) - Not done and is a priority

Sound Scene Type
Agent Secretion (✓) Environment Environmental; one shot
Agent Damage (+) Environment Interface; repeat (plays once every set period for the duration a microbe takes damage)
Agent Inhibition Environment Interface; repeat (plays once every set period for the duration a microbe is inhibited)
Ambience (✓) (needs rework/improvements?) Environment/Editor Interface; continuous
Button Click Unavailable All Interface; one shot
Button Click (✓) All Interface; one shot
Button Hover All Interface; one shot
Disaster Environment Interface; one shot; various types for different disasters, some last longer
Endocytosis Success Environment Interface; one shot
Engulfment (✓) Environment Environmental; one shot
Extinction (Game Over) (✓) Environment Interface; one shot; likely to be somewhat musical in nature
Insufficient Mutation Points (✓) Editor Interface; one shot
Microbe Bonding (+) Environment Environmental; one shot
Microbe Death (✓) Environment Environmental; one shot
Microbe Division (✓) Environment Environmental; one shot
Microbe Multicellular Colony growth Environment Environmental; one shot
Microbe-Microbe Collision (✓) Environment Environmental; one shot; volume scales with impact speed
Microbe-Surface Collision Environment Environmental; one shot; volume scales with impact speed
Movement 1 (✓) Environment Environmental; one shot; plays with substantial change in linear or angular momentum
Movement 2 (✓) Environment Environmental; one shot; plays with substantial change in linear or angular momentum
Movement 3 (✓) Environment Environmental; one shot; plays with substantial change in linear or angular momentum
Multicellular (Victory) Environment Interface; one shot; likely to be somewhat musical in nature
Organelle Collection (✓) Environment Interface; one shot; overridden by endocytosis successful
Pilus Stab (✓) Environment Environmental; one shot
Selection/Placement (✓) Editor Interface; one shot
Selection/Placement Invalid (✓) Editor Interface; one shot; overridden by insufficient Mutation Points sound
Surface-Surface Collision Environment Environmental; one shot; volume scales with impact speed
Ice Shard Damage (✓) Environment Environmental; one shot
Out of ATP Damage (✓) Environment Interface; repeat (plays once every set period for the duration a microbe takes damage)